Chemical purification and analysis
For the isolation and physical/chemical analysis of a wide range of samples
Chromatography: HPLC-Agilent 1260 (x2)
The reversed phase HPLC is an instrument used for the separation, identification and quantification of various components in a mixture via UV-vis detection.
Used for:
- Qualitative analysis
- Quantitative analysis
- Sample purification and separation
Specifications
- Quaternary pump (flow range 0.001-10mL/min)
- Autosampler (Injection volume 0.1-100 uL, 120 positions sample tray)
- Fraction collector
- Mobile phase: Water, Acetonitrile or Methanol (HPLC grade provided by user)
- C18 columns available if needed
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 309
Bookings – only after induction and training
Chromatography: HPLC-Agilent 1290
The reversed phase HPLC is an instrument used for the separation, identification and quantification of various components in a mixture via UV, fluorescence, RID and ELSD detection.
Used for:
- Qualitative analysis
- Quantitative analysis
- Sample purification and separation
Specifications
- Agilent 385 ELSD
- Fraction Collector analytical scale
- Infinity Diode-Array Detector
- Standard Autosampler
- Infinity Autosampler
- Infinity Binary Pump
- Thermostatted Column Compartment
- Infinity Fluorescence DetectorMobile phase: Water, Acetonitrile or Methanol (HPLC grade provided by user)
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
14 Eastern road, room 220 (PC2 lab)
Bookings – only after induction and training
Chromatography: GCMS-Shimadzu QP2010
Gas chromatogram mass spectrometer is an instrument used for the separation, identification and quantification of various components in a mixture via flame ionization and mass detection.
Used for:
- Qualitative analysis
- Quantitative analysis
- Mass analysis
- Only volatile molecules will be detected
Specifications
- Injection mode: Direct, Autosampler (120 positions sample tray), SPME
- Single quadrupole (SIM/scan mode, 10 to 1000 m/z, Electron ionization)
- Flame ionization detector (FID)
- Mobile phase: UHP Helium (column flow rate 10 mL/min)
- Large selection of capillary columns available on demand
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 202
Bookings – only after induction and training
Chromatography: GC-MS/MS-Shimadzu TQ8040
Gas chromatogram mass spectrometer is an instrument used for the separation, identification and quantification of various components in a mixture via flame ionization and mass detection.
Used for:
- Qualitative analysis
- Quantitative analysis
- Mass analysis
- Only volatile molecules will be detected
Specifications
- Injection mode: Direct, Autosampler (120 positions sample tray), SPME, Headspace
- Triple quadrupole (SIM, scan, MRM mode, 10 to 1000 m/z, Electron ionization)
- Flame ionization detector (FID)
- Mobile phase: UHP Helium (column flow rate 10 mL/min)
- Large selection of capillary columns available on demand
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 202
Bookings – only after induction and training
Chromatography: LC/MS-Agilent 1260
The reversed phase LC/MS is an instrument used for the separation, identification and quantification of various components in a mixture via UV-vis and mass detection.
Used for:
- Qualitative analysis
- Quantitative analysis
- Mass analysis
Specifications
- Quaternary pump (flow range 0.001-10mL/min)
- Autosampler (Injection volume 0.1-100 L, 120 positions sample tray)
- Single quadrupole (SIM/scan mode, positive and negative polarity, ESI-API ionization)
- Diode Array Detector (190-640 nm)
- Mobile phase: Water, Acetonitrile or Methanol + 0.1% formic acid (LC/MS grade provided by department)
- C18 columns available if needed
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 311
Elemental Analyser: Elementar®- Vario Micro Cube
Analysis of carbon, hydrogen, nitrogen and sulfur content for a wide range of samples.
Used for: C%, H%, N% and S% in sample
Specifications
- For solid and liquid samples
- Minimum amount of 2 mg per sample for duplicates.
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
10 Sir Christopher Ondaatje Avenue, room 221
Spectrometer: Circular dichroism: Jasco J-810
Circular dichroism (CD) measures the difference in absorbance of left- and right-handed polarized light, which can be a powerful tool for identification and characterisation of chiral molecules. The J-810 is a hybrid instrument consisting of a variable wavelength polarimeter and absorption spectrophotometer (~180-900 nm, Far-UV to Vis range). Our J-810 is also fitted with an Optical Rotation Dispersion (ORD) module and Peltier temperature control unit making it ideally suited to measure optical purity/identification of small molecules and natural products chemistry.
Specifications
- Light source: 150 W Xenon arc lamp (air cooled)
- Wavelength range: ~180 to ~900 nm (standard)
- Four Channel Simultaneous Data Acquisition: Input channels: 2 internal plus 2 external signals can be acquired simultaneously
- ORDM-401 unit fitted
Contact
Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 302 (restricted access)
Spectrometer: Infra-red: Jasco FT/IR-4700
The FT/IR 4700 is a Mid-IR Fourier Transform Infrared (FTIR) spectrometer (wavenumber range 7800 to 350 cm-1) with high resolution (0.4cm-1 and S/N over 35,000:1), making it ideal for transmission measurements of solid, liquid and gas samples. A real-time data processing function can be used to observe and verify the spectrum during measurements.
The FT/IR 4700 can also be paired with the PIKE MIRacle™ Single Reflection ATR accessory with ZeSe crystal for high throughput attenuated total reflectance (ATR) measurements.
Specifications
- Light source: High-intensity ceramic source
- Wavenumber Range: 7,800 to 350 cm-1
- Wavenumber accuracy: ± 0.01 cm-1 (theoretical value)
- Maximum resolution: 0.4 cm-1
- Optical System: Single beam
- Sample chamber: Size: 200 mm (W) × 260 mm (D) × 185 mm (h) (can be nitrogen purged)
Contact
Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 302 (restricted access)
Spectrometer: Microwave Plasma-Atomic Emission Spectrometer: Agilent 4100 MP-AES
Microwave induced Plasma Atomic Emission Spectrophotometer (MP-AES) measures levels of elements in solution.
Used for: simultaneous multi-analyte determination of major and minor elements. Working range of low ppm to weight percent (wt. %).
Specifications
- Nitrogen supplied
- Samples must be digested in low concentrated nitric acid
- A wide range of ICP grade standards available in teaching lab
- Similar to ICP-MS, AAS.
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
14 Sir Christopher Ondaatje Avenue, room 321.
Bookings please contact Dr Remi Rouquette
Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 400 MHz
The Avance 400 MHz NMR spectrometer, with the SampleXpress 60 Holder Autosampler and Iconnmr automation software, is the workhorse of the NMR facility. Samples are conveniently submitted and queued for analysis.
Used for: routine solution state 1D and 2D experiments on small molecules.
Specifications
- 5mm BBFO SmartProbe™ (19F/31P-109Ag)
- Actively-shielded high performance Z-gradient
- 19F{1H} and 2D 1H/19F correlation spectroscopy
- Operating temperature range: -150°C to +150°C
- Ascend 400 MHz (9.4 T) Magnet
- Drift rate < 4.0 Hz/hr
- BCUII (Bruker Chiller Unit)
- Accessible temperatures ≥ 200 K
- SampleXpress 60 Holder Autosampler
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 014 (highly restricted access)
Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 500 MHz
The Avance 500 MHz NMR spectrometer is set up to run variable temperature experiments (-70°C to 100°C) routinely. Very low temperature experiments (-70°C to -150°C) can also be run with use of the liquid nitrogen evaporator and staff assistance. It is highly sensitive for carbon detection, thus it is often utilized to acquire data for the complete characterization of small to medium sized organic molecules.
Used for: variable temperature solution state experiments, characterization experiments
Specifications
- 5mm BBFO SmartProbe™ (19F/31P-109Ag)
- Actively-shielded high performance Z-gradient
- 19F{1H} and 2D 1H/19F correlation spectroscopy
- Operating temperature range: -150°C to +150°C
- Ascend 500 MHz (11.7 T) Magnet
- Drift rate < 5.0 Hz/hr
- Magnet leg upgrade to damp vibrations above 3.8 Hz
- BCUII (Bruker Chiller Unit)
- Accessible temperatures ≥ 200 K
- SampleCase 24 Holder Autosampler
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 014 (highly restricted access)
Spectrometer: Nuclear Magnetic Resonance: Bruker AVII 600 MHz NMR Spectrometer
The AVII 600 MHz NMR spectrometer is equipped with cryoprobe and is utilized for experiments where the upmost of sensitivity is required, such as protein studies and natural product characterisations.
Used for: characterisation of natural products, protein NMR structure determination. Variable temperature work is not permitted.
Specifications
- 5 mm TXI Cryoprobe with Z-gradient (1H/13C/15N/2H observe)
- 3 high power channel operation
- SampleXpress Lite 16 Holder Autosampler
- BCU05 (Bruker Chiller Unit)
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers..
Location
4 Wally’s walk, room 014 (highly restricted access)
Spectrometer: UV-visible : Jasco V-760
The V-760 is a double-beam UV-Visible spectrometer that measures light absorption, reflectance or transmittance in the visible to ultraviolet region (187-900 nm). The extended photometric linearity of the V-760 (up to 6 AU) enables measurement of highly absorbing samples which is particularly useful for materials with low transmission properties such as solid films. The V-760 is also fitted with a 6-position multi-cell changer and Peltier temperature control unit making it an ideal UV-vis instrument for kinetic and thermodynamic experiments.
Specifications
- Optical System: Czerny-Turner mount, double monochromator, fully symmetrical double beam
- Light Source: Halogen lamp, deuterium lamp
- Wavelength range: 187 to 900 nm (+/-0.1 nm at 656.1 nm)
- Photometric Range: -4-6 AU +/-0.3 %T
- Scanning Speed: 10-400 nm/min
Contact
Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally’s walk, room 302 (restricted access)
Thermal analysis: TGA- TA instrument Thermal
Thermogravimetric analysis monitors the weight change of a wide range of samples upon variation of temperature
Used for: mass variation of sample under normal or inert atmosphere.
Specifications
- Heating up to 1000°C
- Aluminum or ceramic pans
- Air or Nitrogen atmosphere
Contact
Dr Aleksei Marianov
4 Wally’s Walk, room 221
Macquarie University
aleksei.marianov@mq.edu.au
T: 02 9850 4219
Rates
Discounted rates apply for Macquarie university researchers.
Location
4 Wally,s Walk, room 202.