Publications

New Publications

August 2024

We have just published that α-Mannosylated HLA-II glycopeptide antigens dominate the immunopeptidome of immortalised cells and tumour tissues. Check it out here: https://doi.org/10.1093/glycob/cwae057

November 2023

We recently published that Protein specific N- and O-glycosylation of the reactive centre loop impacts neutrophil elastase-mediated proteolysis of corticosteroid-binding globulin available in Journal of Biological Chemistry: https://doi.org/10.1016/j.jbc.2023.105519

October 2023

Check out our recent publication where we find that Profound N-glycan remodelling accompanies MHC-II immunopeptide presentation available in Frontiers in Immunology:  https://doi.org/10.3389/fimmu.2023.1258518

July 2023

The legacy project, years in the making  was accepted into PNAS! Congratulations Assoc Prof Rebeca Kawahara and Dr Julian Ugonotti for spearheading this important mapping of glycoproteins in maturing and mature neutrophils. Check it out here: https://doi.org/10.1073/pnas.2303867120

July 2023

Experimentally led by Dr Caroline Carnielli, our new paper on oral cancer glycosylation is now out in Mol Cell Proteomics, a study that was based on a fruitful Australian-Japanese-Brazilian collaboration. https://doi.org/10.1016/j.mcpro.2023.100586.

April 2023

Check out our opinion piece on Critical considerations in N-glycoproteomics in Current Opinions in Chemical Biology https://doi.org/10.1016/j.cbpa.2023.102272

March 2023

Check out the most commonly used experimental approach used in our lab: Glycomics-assisted glycoproteomics enables deep and unbiased N-glycoproteome profiling of complex biological specimens published in Methods in Molecular Biology. https://doi.org/10.1007/978-1-0716-2978-9_16

November 2022

Check out our new review about Glycoproteomics on Nature Reviews Methods Primers. https://doi.org/10.1038/s43586-022-00128-4

March 2022

Check out our new publication: N-acetyl-β-D-hexosaminidases mediate the generation of paucimannosidic proteins via a putative noncanonical truncation pathway in human neutrophils published in Glycobiology. https://doi.org/10.1093/glycob/cwab108

November 2021

Our first Human Glycoproteomics Initiative (HGI) study of glycopeptide data analysis methods conducted over the past four years through the Human Proteome Organization are now available at Nature Methods, Community evaluation of glycoproteomics informatics solutions reveals high-performance search strategies for serum glycopeptide analysis. https://rdcu.be/cABRe

August 2021

Check out our new paper on Oligomannose-cancer associations: Trends in oligomannosylation and α1,2-mannosidase expression in human cancers. https://doi.org/10.18632/oncotarget.28064

May 2021

Check out our new publication: Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells on Cell Reports: https://doi.org/10.1016/j.celrep.2021.109179

February 2021

Check out our new paper on the potential of quantitative serum N-glycomics in Bacteremic Patients Infected with Different Pathogens: Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.
https://doi.org/10.3390/jcm10030516

December 2020

- Check out our comprehensive and first-ever review of the emerging field of glycan structure-focused glycoproteomics published in Biochem Soc Trans: Towards Structure-Focused Glycoproteomics.
https://doi.org/10.1042/BST20200222
- Check out our latest paper on novel aspects of neutrophil MPO glycobiology out in J Biol Chem: Hyper-truncated Asn355- and Asn391-glycans modulate the activity of neutrophil granule myeloperoxidase.
https://doi.org/10.1074/jbc.RA120.016342

October 2020

Check out our new paper on glycobiology aspects of prostate cancer tissue out in Molecular & Cellular Proteomics: The complexity and dynamics of the tissue glycoproteome associated with prostate cancer progression.
https://doi.org/10.1074/mcp.RA120.002320

August 2020

Check out our comprehensive review on Neutrophil Glycobiology recently accepted in Molecular Aspects of Medicine: Structural and functional diversity of neutrophil glycosylation in innate immunity and related disorders.
https://doi.org/10.1016/j.mam.2020.100882

March 2020

High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to- macrophage transition.
Protein glycosylation impacts the development and function of innate immune cells. The glyco-phenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described. Herein, label-free PGC-LC-MS/MS was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition.
Check it out! https://doi.org/10.1093/glycob/cwaa020

August 2019

Protein Paucimannosylation is an Enriched N‐glycosylation Signature of Human Cancers.
This glycomics-centric study explored the association between protein paucimannosylation and a range of human cancer types enabled by access to almost 500 information-rich glycomics datasets collected over a decade using a uniform analytical strategy involving porous graphitised carbon-liquid chromatography-tandem mass spectrometry. This re-interrogation effort, the to-date largest of its kind in glycomics research, demonstrated that paucimannosidic N-glycans are enriched in human cancers.
Check it out! https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.201900010

Human protein paucimannosylation: cues from the eukaryotic kingdoms.
Protein paucimannosylation, an unusual truncated type of N-linked glycosylation of human proteins, was described almost four decades ago, but our knowledge of the structure, function and biosynthesis of this protein modification remains incomplete. With the aim to improve our understanding of the biological roles of paucimannosidic proteins in human glycobiology, we have comprehensively surveyed the literature describing protein paucimannosylation across the eukaryotic kingdoms. In short, this synthetic review finds that human paucimannosidic proteins are biological significant, yet still heavily under-studied biomolecules in human glycobiology that serve essential functions and create structural heterogeneity not dissimilar to other classes of human N-glycoproteins.
Check it out! https://onlinelibrary.wiley.com/doi/10.1111/brv.12548

March 2019

Hinneburg H, Chatterjee S, Schirmeister F, Nguyen-Khuong T, Packer NH, Rapp E, Thaysen-Andersen M. Post-Column Make-up Flow (PCMF) Enhances the Performance of Capillary-Flow PGC-LC-MS/MS-Based Glycomics. Anal Chem. 2019. doi: 10.1021/acs.analchem.8b05720. [Epub ahead of print]
https://www.ncbi.nlm.nih.gov/pubmed/30810297